Master Your Lab Assistant Interview
Comprehensive questions, model answers, and proven strategies to help you shine in any lab setting.
- Real‑world Lab Assistant interview questions
- Step‑by‑step STAR model answers
- Key competencies and evaluation criteria
- Tips to avoid common pitfalls
- Downloadable practice pack
General
During my internship at XYZ University’s chemistry lab, I was part of a team handling daily sample analyses.
My responsibility was to prepare reagents, operate analytical instruments, and record results accurately.
I followed SOPs for reagent preparation, calibrated equipment before each use, and maintained a detailed electronic log of all measurements.
The lab achieved a 98% on-time report delivery rate, and my supervisor praised my meticulous record‑keeping, which reduced data entry errors by 15%.
- How did you handle a situation when an instrument malfunctioned?
- Can you give an example of a challenging sample you processed?
- Clarity of role and responsibilities
- Use of specific lab techniques
- Quantifiable results
- STAR structure
- Vague description of duties
- No mention of lab safety or accuracy
- Internship at university chemistry lab
- Prepared reagents and operated instruments
- Followed SOPs and logged data meticulously
- Improved report timeliness and reduced errors
I have followed your company's recent breakthroughs in biomedical research, especially the development of rapid diagnostic kits.
I want to contribute my lab skills to support those projects and grow within a cutting‑edge environment.
I researched your lab’s workflow, identified how my experience with assay validation aligns with your needs, and prepared a short presentation on how I could streamline sample prep.
I believe my proactive approach would help reduce turnaround time by up to 10%, supporting faster product development.
- What specific techniques are you most excited to apply here?
- How do you stay updated on industry trends?
- Demonstrates genuine interest
- Links personal experience to company goals
- Shows initiative
- Generic answer without company specifics
- Over‑emphasis on personal gain
- Admire company’s innovations
- Align personal skills with company needs
- Demonstrated proactive research and ideas
Technical Skills
In my previous role at a clinical diagnostics lab, precise pipetting was critical for assay reliability.
I needed to maintain consistent volumes across hundreds of samples daily.
I calibrated pipettes weekly, used reverse‑pipetting for viscous liquids, and recorded calibration logs in the LIMS. I also performed a gravimetric check every shift to verify volume accuracy.
These practices reduced pipetting variance to <0.5% and contributed to a 12% increase in assay reproducibility.
- What steps would you take if a pipette failed calibration?
- How do you handle high‑throughput pipetting tasks?
- Understanding of calibration procedures
- Use of verification methods
- Impact on data quality
- No mention of verification or documentation
- Weekly pipette calibration
- Reverse‑pipetting for viscous liquids
- Gravimetric verification each shift
- Documented in LIMS
While supporting a microbiology lab, I was responsible for maintaining the pH meter used for media preparation.
Ensure the meter provided accurate readings before each batch of media was made.
I rinsed the electrode with distilled water, performed a two‑point calibration using standard buffers at pH 4.00 and 7.00, recorded the slope and offset, and stored the electrode in a protective solution. I logged the calibration in the lab notebook and verified accuracy with a third buffer.
The calibrated meter consistently stayed within ±0.02 pH units, preventing media pH drift that could have compromised microbial growth.
- What would you do if the meter failed to stabilize?
- How often should calibration be performed for critical assays?
- Step‑by‑step calibration process
- Reason for calibration (accuracy, reproducibility)
- Safety handling of electrodes
- Skipping verification step
- No mention of documentation
- Rinse electrode
- Two‑point calibration with pH 4 and 7 buffers
- Record slope/offset
- Verify with third buffer
- Log results
Safety & Compliance
During a routine inventory check, I noticed that a bottle of concentrated sulfuric acid was stored on a lower shelf without a secondary containment tray.
Prevent a potential spill that could damage equipment and endanger personnel.
I immediately secured the bottle, placed it in a proper acid‑resistant tray, updated the storage map, and reported the incident to the safety officer. I also organized a brief refresher on chemical storage for the team.
No incident occurred, and the safety audit the following month gave the lab a perfect score for chemical storage compliance.
- How do you stay aware of evolving safety regulations?
- What would you do if a colleague ignored your safety recommendation?
- Prompt hazard identification
- Correct corrective actions
- Communication with safety personnel
- Follow‑up training
- Blaming others
- Lack of concrete actions
- Identified improperly stored acid
- Secured bottle and added containment
- Updated storage documentation
- Reported to safety officer
- Conducted team refresher
In my role at a microbiology research facility, we generated biohazardous waste daily.
Ensure waste is disposed of safely and in compliance with biosafety regulations.
I segregated waste into autoclave‑compatible bags, labeled them with biohazard symbols, logged each batch in the waste tracking system, and arranged for scheduled autoclave cycles before final disposal to the approved incinerator.
Our lab maintained a zero‑incident record for biohazard exposure and passed all external biosafety inspections.
- How would you handle a spill of biohazardous material?
- What documentation is required for regulatory audits?
- Knowledge of segregation, labeling, decontamination
- Compliance tracking
- Regulatory awareness
- Omitting autoclave step
- Unclear on documentation
- Segregate waste into designated containers
- Label with biohazard symbols
- Log waste in tracking system
- Autoclave before disposal
- Use approved incineration
Teamwork & Communication
A senior researcher reported inconsistent ELISA results for a cytokine assay.
Identify the source of variability and restore assay reliability.
I reviewed the protocol with the researcher, observed the plate washing technique, and discovered that the wash buffer was not being mixed properly. Together we adjusted the mixing procedure, recalibrated the plate reader, and ran a validation set of standards.
The assay CV dropped from 18% to 5%, and the researcher was able to publish the data with confidence.
- What communication strategies help when troubleshooting under time pressure?
- How do you document changes made during troubleshooting?
- Collaboration approach
- Technical insight
- Outcome quantification
- Taking sole credit
- Lack of measurable improvement
- Reviewed protocol with researcher
- Observed washing technique
- Identified improperly mixed buffer
- Adjusted procedure and recalibrated reader
- Validated improved assay performance
In a high‑throughput lab, I often received overlapping sample batches for PCR, spectrophotometry, and cell culture assays.
Create a clear priority order to meet all deadlines without compromising quality.
I first assessed each task’s deadline and impact on downstream projects, then used a digital Kanban board to allocate time blocks, communicated the schedule to the team, and flagged any resource conflicts for the supervisor’s input.
All experiments were completed on schedule, and the lab reported a 10% increase in throughput due to the organized workflow.
- What tools do you use for task management?
- How do you handle unexpected urgent requests?
- Prioritization logic
- Use of organizational tools
- Team communication
- No systematic approach
- Ignoring deadlines
- Assess deadlines and project impact
- Use Kanban board for visual task management
- Communicate schedule to team
- Escalate resource conflicts
- Achieve on‑time completion
- lab techniques
- sample preparation
- safety protocols
- data entry
- equipment maintenance