Ace Your Biology Interview
Master the questions hiring managers love and showcase your scientific expertise
- Real‑world behavioral and technical questions
- STAR‑formatted model answers
- Competency‑based evaluation criteria
- Ready‑to‑use practice pack with timed rounds
General Biology
During my undergraduate genetics course, I was asked to present the flow of genetic information.
Explain the central dogma clearly to classmates with varied backgrounds.
I described how DNA is transcribed into RNA, which is then translated into protein, emphasizing the unidirectional flow and exceptions such as reverse transcription in retroviruses.
My peers grasped the concept, and the professor highlighted my explanation as a model for the class.
- How do retroviruses challenge the central dogma?
- What are the implications of the dogma for gene therapy?
- Accuracy of each step
- Clarity of explanation
- Mention of exceptions
- Relevance to broader biological context
- Confusing DNA/RNA roles
- Omitting translation step
- Overly vague
- DNA → RNA (transcription)
- RNA → Protein (translation)
- Key enzymes: RNA polymerase, ribosome
- Exceptions: reverse transcription, RNA editing
- Why it matters: links genotype to phenotype
In a lab interview, the panel asked me to compare cell types quickly.
Provide a concise yet comprehensive comparison.
I listed structural, genetic, and metabolic distinctions, using a table format in my mind.
The interviewers noted my organized response and moved on to deeper questions.
- Why does compartmentalization matter for cellular regulation?
- Give an example of a prokaryote that performs a eukaryote‑like function.
- Coverage of key categories
- Correct terminology
- Logical ordering
- Leaving out nucleus or organelles
- Mixing up size ranges
- Nucleus: absent in prokaryotes, present in eukaryotes
- DNA organization: circular plasmid vs. linear chromosomes
- Organelles: no membrane‑bound organelles vs. mitochondria, ER, Golgi, etc.
- Size: 1‑5 µm vs. 10‑100 µm
- Reproduction: binary fission vs. mitosis/meiosis
- Transcription/translation: coupled vs. separated
Research Skills
During my senior thesis, I wanted to assess the impact of invasive plant species on native pollinator visitation rates.
Design a field experiment that isolates the effect of the invasive species while controlling for confounding variables.
I selected paired plots (invaded vs. non‑invaded) across three habitats, standardized flower abundance, used timed observations for pollinator visits, and randomized plot order each day to reduce observer bias.
Statistical analysis (ANOVA) showed a 35% reduction in pollinator visits in invaded plots (p<0.01), supporting my hypothesis and earning a departmental award.
- How would you modify the design if weather variability was high?
- What limitations did you encounter and how did you address them?
- Clarity of hypothesis
- Appropriateness of controls
- Statistical rigor
- Reflection on limitations
- Vague description of controls
- No mention of replication or statistics
- Define clear, testable hypothesis
- Select appropriate control and treatment groups
- Standardize extraneous variables (e.g., flower density)
- Randomize sampling order
- Choose suitable statistical test
- Interpret results in ecological context
In a lab project on enzyme kinetics, my data showed no increase in reaction rate with substrate concentration, contrary to Michaelis‑Menten expectations.
Determine why the results differed and decide next steps.
I re‑checked reagent concentrations, calibrated the spectrophotometer, consulted literature for possible inhibitor presence, and repeated the assay with fresh reagents.
The issue traced to a contaminated substrate; after correction, the expected kinetic curve reappeared. I documented the troubleshooting process in the lab notebook and presented it to the team as a learning case.
- Can you give an example where the unexpected result led to a new discovery?
- How do you communicate such setbacks to supervisors?
- Systematic troubleshooting approach
- Use of controls
- Willingness to revise hypothesis
- Clear communication
- Blaming external factors without verification
- Skipping replication
- Verify experimental setup and reagents
- Check instrument calibration
- Review literature for alternative explanations
- Repeat experiment with controls
- Document findings and adjust hypothesis if needed
Data Analysis
For a collaborative project, I received raw FASTQ files from an RNA‑seq experiment comparing treated vs. control cells.
Process, normalize, and identify differentially expressed genes using appropriate statistical methods.
I used FastQC for quality checks, trimmed adapters with Trimmomatic, aligned reads with STAR, generated count matrices with featureCounts, imported data into DESeq2 in R, performed variance stabilizing transformation, and applied the Wald test with Benjamini‑Hochberg correction.
The analysis revealed 1,200 up‑regulated and 950 down‑regulated genes (adjusted p<0.05), which we validated by qPCR for key targets.
- What challenges arise with low‑count genes?
- How would you visualize the results for a non‑technical audience?
- Correct pipeline steps
- Understanding of normalization
- Statistical test justification
- Awareness of multiple testing
- Skipping QC or alignment verification
- Misidentifying the statistical test
- Quality control (FastQC)
- Read trimming (Trimmomatic)
- Alignment (STAR)
- Count generation (featureCounts)
- Import to DESeq2
- Normalization (VST)
- Differential expression testing
- Multiple testing correction
At a community science fair, I needed to explain my research on antibiotic resistance to high school students and their parents.
Translate technical results into an engaging, understandable story.
I created a short animated video using analogies (e.g., bacteria as 'invaders' and antibiotics as 'defense weapons'), highlighted key findings with simple graphs, and used everyday language to describe mechanisms.
Visitors spent extra time at my booth, asked follow‑up questions, and several parents expressed interest in supporting local stewardship programs.
- How do you gauge whether your audience understood the material?
- What adjustments would you make for a senior‑level policy briefing?
- Clarity of language
- Use of analogies
- Audience engagement
- Effectiveness of visual aids
- Over‑technical language
- Lack of audience focus
- Identify core message
- Choose relatable analogies
- Simplify data visualizations
- Avoid jargon
- Engage with interactive elements
- molecular biology
- experimental design
- data analysis
- PCR
- cell culture
- bioinformatics
- hypothesis testing