INTERVIEW

Ace Your Biology Interview

Master the questions hiring managers love and showcase your scientific expertise

6 Questions
90 min Prep Time
5 Categories
STAR Method
What You'll Learn
To equip aspiring biologists with targeted interview questions, model answers, and actionable preparation tools that align with industry expectations.
  • Real‑world behavioral and technical questions
  • STAR‑formatted model answers
  • Competency‑based evaluation criteria
  • Ready‑to‑use practice pack with timed rounds
Difficulty Mix
Easy: 0.4%
Medium: 0.4%
Hard: 0.2%
Prep Overview
Estimated Prep Time: 90 minutes
Formats: behavioral, technical, case study
Competency Map
Scientific Knowledge: 25%
Experimental Design: 20%
Data Interpretation: 20%
Communication: 20%
Problem Solving: 15%

General Biology

Can you explain the central dogma of molecular biology and its significance?
Situation

During my undergraduate genetics course, I was asked to present the flow of genetic information.

Task

Explain the central dogma clearly to classmates with varied backgrounds.

Action

I described how DNA is transcribed into RNA, which is then translated into protein, emphasizing the unidirectional flow and exceptions such as reverse transcription in retroviruses.

Result

My peers grasped the concept, and the professor highlighted my explanation as a model for the class.

Follow‑up Questions
  • How do retroviruses challenge the central dogma?
  • What are the implications of the dogma for gene therapy?
Evaluation Criteria
  • Accuracy of each step
  • Clarity of explanation
  • Mention of exceptions
  • Relevance to broader biological context
Red Flags to Avoid
  • Confusing DNA/RNA roles
  • Omitting translation step
  • Overly vague
Answer Outline
  • DNA → RNA (transcription)
  • RNA → Protein (translation)
  • Key enzymes: RNA polymerase, ribosome
  • Exceptions: reverse transcription, RNA editing
  • Why it matters: links genotype to phenotype
Tip
Use a simple diagram or analogy (e.g., blueprint → instruction manual → building) to illustrate the flow.
What are the main differences between prokaryotic and eukaryotic cells?
Situation

In a lab interview, the panel asked me to compare cell types quickly.

Task

Provide a concise yet comprehensive comparison.

Action

I listed structural, genetic, and metabolic distinctions, using a table format in my mind.

Result

The interviewers noted my organized response and moved on to deeper questions.

Follow‑up Questions
  • Why does compartmentalization matter for cellular regulation?
  • Give an example of a prokaryote that performs a eukaryote‑like function.
Evaluation Criteria
  • Coverage of key categories
  • Correct terminology
  • Logical ordering
Red Flags to Avoid
  • Leaving out nucleus or organelles
  • Mixing up size ranges
Answer Outline
  • Nucleus: absent in prokaryotes, present in eukaryotes
  • DNA organization: circular plasmid vs. linear chromosomes
  • Organelles: no membrane‑bound organelles vs. mitochondria, ER, Golgi, etc.
  • Size: 1‑5 µm vs. 10‑100 µm
  • Reproduction: binary fission vs. mitosis/meiosis
  • Transcription/translation: coupled vs. separated
Tip
Structure your answer as a side‑by‑side list for easy recall.

Research Skills

Describe a time you designed an experiment to test a hypothesis in ecology.
Situation

During my senior thesis, I wanted to assess the impact of invasive plant species on native pollinator visitation rates.

Task

Design a field experiment that isolates the effect of the invasive species while controlling for confounding variables.

Action

I selected paired plots (invaded vs. non‑invaded) across three habitats, standardized flower abundance, used timed observations for pollinator visits, and randomized plot order each day to reduce observer bias.

Result

Statistical analysis (ANOVA) showed a 35% reduction in pollinator visits in invaded plots (p<0.01), supporting my hypothesis and earning a departmental award.

Follow‑up Questions
  • How would you modify the design if weather variability was high?
  • What limitations did you encounter and how did you address them?
Evaluation Criteria
  • Clarity of hypothesis
  • Appropriateness of controls
  • Statistical rigor
  • Reflection on limitations
Red Flags to Avoid
  • Vague description of controls
  • No mention of replication or statistics
Answer Outline
  • Define clear, testable hypothesis
  • Select appropriate control and treatment groups
  • Standardize extraneous variables (e.g., flower density)
  • Randomize sampling order
  • Choose suitable statistical test
  • Interpret results in ecological context
Tip
Mention sample size, replication, and how you ensured data reliability.
How do you handle unexpected results that contradict your hypothesis?
Situation

In a lab project on enzyme kinetics, my data showed no increase in reaction rate with substrate concentration, contrary to Michaelis‑Menten expectations.

Task

Determine why the results differed and decide next steps.

Action

I re‑checked reagent concentrations, calibrated the spectrophotometer, consulted literature for possible inhibitor presence, and repeated the assay with fresh reagents.

Result

The issue traced to a contaminated substrate; after correction, the expected kinetic curve reappeared. I documented the troubleshooting process in the lab notebook and presented it to the team as a learning case.

Follow‑up Questions
  • Can you give an example where the unexpected result led to a new discovery?
  • How do you communicate such setbacks to supervisors?
Evaluation Criteria
  • Systematic troubleshooting approach
  • Use of controls
  • Willingness to revise hypothesis
  • Clear communication
Red Flags to Avoid
  • Blaming external factors without verification
  • Skipping replication
Answer Outline
  • Verify experimental setup and reagents
  • Check instrument calibration
  • Review literature for alternative explanations
  • Repeat experiment with controls
  • Document findings and adjust hypothesis if needed
Tip
Emphasize a methodical, data‑driven approach rather than jumping to conclusions.

Data Analysis

Explain how you would use statistical software to analyze a gene expression dataset from RNA‑seq.
Situation

For a collaborative project, I received raw FASTQ files from an RNA‑seq experiment comparing treated vs. control cells.

Task

Process, normalize, and identify differentially expressed genes using appropriate statistical methods.

Action

I used FastQC for quality checks, trimmed adapters with Trimmomatic, aligned reads with STAR, generated count matrices with featureCounts, imported data into DESeq2 in R, performed variance stabilizing transformation, and applied the Wald test with Benjamini‑Hochberg correction.

Result

The analysis revealed 1,200 up‑regulated and 950 down‑regulated genes (adjusted p<0.05), which we validated by qPCR for key targets.

Follow‑up Questions
  • What challenges arise with low‑count genes?
  • How would you visualize the results for a non‑technical audience?
Evaluation Criteria
  • Correct pipeline steps
  • Understanding of normalization
  • Statistical test justification
  • Awareness of multiple testing
Red Flags to Avoid
  • Skipping QC or alignment verification
  • Misidentifying the statistical test
Answer Outline
  • Quality control (FastQC)
  • Read trimming (Trimmomatic)
  • Alignment (STAR)
  • Count generation (featureCounts)
  • Import to DESeq2
  • Normalization (VST)
  • Differential expression testing
  • Multiple testing correction
Tip
Mention both the biological interpretation and the reproducibility of the workflow.
Give an example of how you communicated complex scientific findings to a non‑expert audience.
Situation

At a community science fair, I needed to explain my research on antibiotic resistance to high school students and their parents.

Task

Translate technical results into an engaging, understandable story.

Action

I created a short animated video using analogies (e.g., bacteria as 'invaders' and antibiotics as 'defense weapons'), highlighted key findings with simple graphs, and used everyday language to describe mechanisms.

Result

Visitors spent extra time at my booth, asked follow‑up questions, and several parents expressed interest in supporting local stewardship programs.

Follow‑up Questions
  • How do you gauge whether your audience understood the material?
  • What adjustments would you make for a senior‑level policy briefing?
Evaluation Criteria
  • Clarity of language
  • Use of analogies
  • Audience engagement
  • Effectiveness of visual aids
Red Flags to Avoid
  • Over‑technical language
  • Lack of audience focus
Answer Outline
  • Identify core message
  • Choose relatable analogies
  • Simplify data visualizations
  • Avoid jargon
  • Engage with interactive elements
Tip
Test your explanation on a colleague outside your field before the actual presentation.
ATS Tips
  • molecular biology
  • experimental design
  • data analysis
  • PCR
  • cell culture
  • bioinformatics
  • hypothesis testing
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Timed Rounds: 45 minutes
Mix: easy, medium, hard

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